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Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)

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商品描述

Product name

Anti-alpha smooth muscle Actin antibody [4A4]
See all alpha smooth muscle Actin primary antibodies
Description

Mouse monoclonal [4A4] to alpha smooth muscle Actin
Host species

Mouse
Tested applications

Suitable for: WB,ELISA,IHC-P,Flow Cyt,ICC/IF
Species reactivity

Reacts with: Mouse, Rat, Human, Monkey
Immunogen

Synthetic peptide of Human alpha smooth muscle Actin (C-EYDEAGPSIVHRKCF) conjugated KLH
Positive control
- Recombinant alpha smooth muscle Actin protein, Hela, A431, Jurkat, K562, HEK293, HepG2, NIH3T3, PC12 and COS7 cell lysates, HepG2 cells, HeLa cells

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration
- 100 μl at 1 mg/ml
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

4A4
Isotype

IgG1

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)

Immunohistochemical analysis of paraffin-embedded Human duodenum tissue using ab119952 at a dilution of 1/200 with DAB staining.
Western blot - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)

All lanes : Anti-alpha smooth muscle Actin antibody [4A4] (ab119952) at 1/500 dilution

Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : HepG2 cell lysate
Lane 7 : NIH3T3 cell lysate
Lane 8 : PC12 cell lysate
Lane 9 : COS7 cell lysate

Predicted band size: 42 kDa
Observed band size: 42 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)

Immunohistochemical analysis of paraffin-embedded Human esophagus tissue using ab119952 at a dilution of 1/200 with DAB staining.
Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)

Immunofluorescence analysis of HepG2 cells using ab119952 at 1/200 (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (Red). DRAQ5 fluorescent DNA dye (blue).
Flow Cytometry - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)

Flow cytometric analysis of Hela cells using ab119952 at a dilution of 1/200 (green) and negative control (purple).
ELISA - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)

ELISA, using ab119952 at a dilution of 1/10000.